Composite

Part:BBa_K5082011:Design

Designed by: Fangyuan Duan   Group: iGEM24_YiYe-China   (2024-09-07)


pCMV-EGFP-EIF3B-HSU


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1729
    Illegal XbaI site found at 1766
    Illegal SpeI site found at 315
    Illegal PstI site found at 1734
    Illegal PstI site found at 3088
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1729
    Illegal NheI site found at 961
    Illegal SpeI site found at 315
    Illegal PstI site found at 1734
    Illegal PstI site found at 3088
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1729
    Illegal BglII site found at 1709
    Illegal BglII site found at 6215
    Illegal BamHI site found at 1760
    Illegal XhoI site found at 1713
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1729
    Illegal XbaI site found at 1766
    Illegal SpeI site found at 315
    Illegal PstI site found at 1734
    Illegal PstI site found at 3088
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1729
    Illegal XbaI site found at 1766
    Illegal SpeI site found at 315
    Illegal PstI site found at 1734
    Illegal PstI site found at 3088
    Illegal NgoMIV site found at 2198
    Illegal NgoMIV site found at 3539
    Illegal NgoMIV site found at 3822
    Illegal AgeI site found at 970
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 948
    Illegal BsaI.rc site found at 5347
    Illegal SapI site found at 4267
    Illegal SapI.rc site found at 3388
    Illegal SapI.rc site found at 3598


Design Notes

1


Source

1

References